2016 SIMB Annual Meeting and Exhibition

Sugar synthesis from CO2 in E. coli
David Wernick, Niv Antonovsky, Shmuel Glazier, Elad Noor, Yehudit Zohar, Elard Herz, Uri Barenholz, Dan Davidi, Yinon Bar-On, Arren Barr-Even and Ron Milo, Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rhhovot, Israel

Pathway compartmentalization in peroxisome of saccharomyces cerevisiae to produce versatile medium chain fatty alcohols
Jiayuan Sheng, Xueyang Feng and Joseph Stevens, Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA

Engineering of hydroxytyrosol production in Escherichia coli
Taek Soon Lee, Erika Yoshida and Daisuke Koma, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Emeryville, CA

Influence of heterologous 2,3-butanediol pathway engineering and oxygen level on host metabolism of Zymomonas mobilis
Shihui Yang¹, Ali Mohagheghi¹, Mary Ann Franden¹, Yat-Chen Chou¹, Xiaowen Chen¹, Nancy Dowe¹, Michael E. Himmel² and Min Zhang¹, (1)National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO, (2)Biosciences Center, National Renewable Energy Laboratory, Golden, CO

Membrane damage during production of biorenewable fuels and chemicals and compensatory membrane engineering strategies
Laura R. Jarboe¹, Zaigao Tan¹, Jieni Lian², Tao Jin¹, Yingxi Chen¹ and Jacqueline Shanks¹, (1)Chemical and Biological Engineering, Iowa State University, Ames, IA, (2)Bioeconomy Institute, Iowa State University, Ames, IA

Understanding the role of the hfs hydrogenase in Thermoanaerobacterium saccharolyticum
Ayşenur Eminoğlu, Biology, Recep Tayyip Erdogan University, Rize, Turkey, Daniel Olson, Thayer School of Engineering, Dartmouth College, Hanover, NH and Prof. Lee R. Lynd, BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN

Development of metabolic engineering toolbox for lipid production in oleaginous red yeast, Sporidiobolus pararoseus
Yelena Betz¹, Allatah Mekile¹, Liang Wu², Leonie Bonder-van Dijk², Tom Goldman³, David Simpson¹, Adam Burja¹ and Ross Zirkle¹, (1)DSM Nutritional Products, Columbia, MD, (2)DSM Biotechnology Center, Delft, Netherlands, (3)Biosciences Center, BP Group Technology, San Diego, CA

Reliable and predictable gene expression tools for Pseudomonas putida
Wasti Nurani, Taylor Cook, Jacqueline Rand, Fransiskus Ivan, Yalun Arifin and Brian Pfleger, Chemical and Biological Engineering, University of Wisconsin - Madison, Madison, WI

Engineering Escherichia coli for the synthesis of short- and medium-chain α,β-unsaturated carboxylic acids
Seohyoung Kim, Chemical and Biomolecular Engineering, Rice university, Houston, TX, Seokjung Cheong, Rice University, Houston, TX and Ramon Gonzalez, Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX

Escherichia coli enoyl-acyl carrier protein reductase (FabI) supports efficient operation of a functional reversal of the β-oxidation cycle
Alexander Chou, James M Clomburg, Seohyoung Kim and Ramon Gonzalez, Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX

Modification of butyrate and butanol production in Clostridium acetobutylicum by introduction of a ferredoxin oxidoreductase
David Magnan¹, Dr. Chandresh Thakker², Ka-Yiu San³ and George N. Bennett², (1)Department of Bioengineering, Rice University, Houston, TX, (2)Department of Biochemistry and Cell Biology, Rice University, Houston, TX, (3)Department of Bioengineering, Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX

Dual targeting of polyamine (PA) metabolism by inhibiting fungal PA biosynthesis and transport as a strategy to control Aspergillus flavus infection and aflatoxin contamination in corn
Rajtilak Majumdar, Brian Mack, Dr. Matthew Gilbert, Christine Sickler, Dr. Kanniah Rajasekaran, Deepak Bhatnagar and Dr. Jeffrey Cary, USDA, New Orleans, LA

Examining the effects of alternative electron transport in cyanobacteria
Travis Korosh, Department of Environmental Chemistry and Technology, University of Wisconsin-Madison, Madison, WI, Andrew L. Markley, Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI and Brian Pfleger, Chemical and Biological Engineering, University of Wisconsin - Madison, Madison, WI

Genomic hot spots for strong and stable expression sites in Yarrowia lipolytica
Murtaza Shabbir Hussain, Phillip Baker and Mark Blenner, Chemical & Biomolecular Engineering, Clemson University, Clemson, SC

Lactobacillus casei as a biocatalyst for the production of isobutanol
Elena Vinay Lara¹, Ekkarat Phrommao¹, Jeff Broadbent² and James Steele¹, (1)Food Science, University of Wisconsin-Madison, Madison, WI, (2)Nutrition, Dietetics and Food Science, Utah State University, Logan, UT

Re-engineered β-oxidation reversal for the synthesis of ω-fuctionalized carboxylic acids in Escherichia coli
Seokjung Cheong, James M Clomburg and Ramon Gonzalez, Rice University, Houston, TX

Dynamic two-phase fermentation in S. cerevisiae allows for rapid and scalable strain design for microbial chemical synthesis.
Jonathon Burg, Benjamin Reed and Michael Lynch, Biomedical Engineering, Duke University, Durham, NC

Temperature sensitive mutant of the broad host range plasmid pBBR1MCS-2
Jianzhong Huang¹, Xianzhang Jiang¹ and Prof. Yong Tao², (1)College of Life Sciences, Fujian Normal University, Fuzhou, Fujian, China, (2)Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

The toxicity of model lignin monomers to Clostridium thermocellum metabolism
Tobin J. Verbeke, Richard J. Giannone, Nancy L. Engle, Timothy J. Tschaplinski, Robert L. Hettich and James G. Elkins, BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN

Modification of the mannitol biosynthetic pathway in Aureobasidium pullulans to alter the structure of the polyol lipid liamocin
Christopher D. Skory¹, Neil PJ Price¹, Timothy D. Leathers¹, Pennapa Manitchotpisit², Ronald E. Hector³, Michael J. Bowman³ and Joseph O. Rich¹, (1)Renewable Product Technology, United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, IL, (2)School of Biological Sciences, Illinois State University, Normal, IL, (3)Bioenergy Research Unit, United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, IL

Improved genetic tools for rapid engineering of Pseudomonas putida
Joshua Elmore, Anna Furches and Adam Guss, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN

Metabolic engineering of Saccharomyces cerevisiae for the production of sesquiterpenes
Mary Bosserman¹, Lauren Esser¹, M. Braden Wenndt¹, Philippe Prochasson¹, Dayal Saran¹, Sung Park² and Bryan Julien¹, (1)Molecular Biology, Evolva, Inc., Lexington, KY, (2)Fermentation, Evolva, Inc., Lexington, KY

Metabolic inhibition of Clostridium thermocellum by pentose sugars
Tobin J. Verbeke, Richard J. Giannone, Dawn M. Klingeman, Nancy L. Engle, Timothy Tschaplinski, Steven D. Brown, Robert L. Hettich and James G. Elkins, Biosciences Division and BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN

Understanding nitrogen assimilation in Clostridium thermocellum for bioengineering
Thomas Rydzak¹, David Garcia¹, Margaret Sladek¹, Dawn M. Klingeman², Evert K. Holwerda³, Steven D. Brown² and Adam Guss¹, (1)Biosciences Division and BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, (2)Biosciences Division and BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge National Laboratory, Oak Ridge, TN, (3)Thayer School of Engineering, Dartmouth College, Hanover, NH

Site-specific recombinases for integration of heterologous DNA in E. coli
Lauren Riley¹, Carly Duffy², Rebecca O'toole², Beth Papanek² and Dr. Adam M. Guss¹, (1)BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, (2)Biological Sciences, Oak Ridge National Laboratory, Oak Ridge, TN

Production of minor ginsenosides using identified novel ginseng UDP-glycosyltransferases in Saccharomyces cerevisiae
Suk-Chae Jung¹, Dr. Jun Hyoung Lee², Soohwan Lim¹, Dr. Bong Kwan Han¹, Min Sung Kim², Prof. Giltsu Choi³ and Prof. Sun Chang Kim¹, (1)Intelligent Synthetic Biology Center, Korea Advanced Institute of Science and Technology, Daejeon, South Korea, (2)KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Daejeon, South Korea, (3)Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea

Regulatory landscape for microorganisms used in industrial applications in Canada
Souad El Ouakfaoui, Science and Technologie Branch, Environment and climate Change Canada, Gatineau, QC, Canada

Survival strategies of Pseudomonas putida KT2440 during desiccation stress
Antonino Baez, Lesther Lopez-Cruz, Marisol Vazquez-Quiroz and Jesus Munoz-Rojas, Benemerita Universidad Autonoma de Puebla, Puebla, Mexico

Comparative genomic studies of Penicillium spp. that cause blue mold of apple fruit in storage reveal genetic features likely involved in virulence and mycotoxin production
Guangxi Wu¹, Guohua Yin², Jiujiang Yu³, Wayne M. Jurick II³, Joan W. Bennett² and Daniel Shelton³, (1)Oak Ridge Institute for Science and Education, College Park, MD, (2)Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ, (3)Beltsville Agricultural Research Center, USDA/ARS, Beltsville, MD

Evaluation of chard yield using digestate
Myrna Solís, Centro de Investigacion en Biotecnologia Aplicada, INSTITUTO POLITECNICO NACIONAL, Tepetitla, Mexico, Marilyn Checa, Ingenieria Quimica, INSTITUTO TECNOLOGICO DE TLAXCO, Tlaxcala, Mexico and Aida Solis, UNIVERSIDAD AUTONOMA METROPOLITANA UNIDAD XOCHIMILCO, Ciudad de Mexico, Mexico

Rhamnolipids produced using a byproduct of biodiesel industry as stabilizing agents in silver nanoparticles formation
Ana Maria Salazar Bryam¹, Vinícius Luiz da Silva², Roberta Barros Lovaglio³, Cinthia Cristine Moura¹, Reinaldo Marchetto⁴ and Jonas Contiero¹, (1)Bioquimica e Microbiologia, UNESP, Rio Claro, Brazil, (2)Bioquímica e Microbiologia, Universidade Estadual Paulista, Rio Claro, Brazil, (3)Biochemistry and Microbiology, Universidade Estadual Paulista, Rio Claro, Brazil, (4)Bioquímica e Tecnologia Química, Instituto de Química de Araraquara, Araraquara, Brazil

Insecticidal activity and repellency of rhamnolipids produced by Pseudomonas aeruginosa LBI 2A1  against the larvae of Aedes aegypti.
Vinícius Luiz Silva¹, Roberta Barros Lovaglio², Ana Maria Salazar Bryam³, Claudio Von Zuben⁴ and Jonas Contiero³, (1)Bioquímica e Microbiologia, UNiversidade Estadual Paulista, Rio Claro, Brazil, (2)Biochemistry and Microbiology, Universidade Estadual Paulista, Rio Claro, Brazil, (3)Bioquimica e Microbiologia, UNESP, Rio Claro, Brazil, (4)Zoologia, Unesp -Univ. Estadual Paulista, Rio Claro, Brazil

Investigation of volatile-based microbial control for mitigating fungal pathogenicity
Kyle T. Gabriel¹, Christopher T. Cornelison¹, Tom Tomasi², Kevin Keel³ and Sidney A. Crow Jr.¹, (1)Applied and Environmental Microbiology, Georgia State University, Atlanta, GA, (2)Department of Biology, Missouri State University, Springfield, MO, (3)Pathology, Microbiology & Immunology, University of California, Davis, Davis, CA

Biological phosphorous removal from surface waters
Carina Jung, Jed Eberly and Dr. Heather Smith, Environmental Laboratory, US Army Engineer Research & Development Center, Vicksburg, MS

Evaluation of the co-digestion of fruit and vegetable residues with cow and pig manure
Myrna Solís¹, Edgar E. Bustos², Aida Solis³, Erik Ocaranza² and Lilia Tapia², (1)Centro de Investigacion en Biotecnologia Aplicada, INSTITUTO POLITECNICO NACIONAL, Tepetitla, Mexico, (2)Centro de Investigación en Biotecnología Aplicada, INSTITUTO POLITECNICO NACIONAL, Tepetitla, Mexico, (3)UNIVERSIDAD AUTONOMA METROPOLITANA UNIDAD XOCHIMILCO, Ciudad de Mexico, Mexico

Metagenomic analysis of denitrifying wastewater enrichment cultures able to transform the explosive, 3-nitro-1,2,4-triazol-5-one (NTO)
Karl Indest, Jed Eberly, Dawn Hancock, Carina Jung and Fiona Crocker, Environmental Laboratory, US Army Engineer Research & Development Center, Vicksburg, MS

Regulation of mammalian gene expression by bioactive compounds from Cinnamon bark
Heping Cao and Kandan Sethumadhavan, Commodity Utilization, Southern Regional Research Center, ARS, USDA, New Orleans, LA

In vivo and In vitro reconstitution of the dehydroalanine biosynthesis in goadsporin, a linear azole containing RiPP
Hiroyasu Onaka¹, Dr. Shumpei Asamizu¹, Dr. Taro Ozaki¹, Dr. Yuki Goto², Prof. Hiroaki Suga² and Ms. Kona Yamashita¹, (1)Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan, (2)Depertment of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo, Japan

Biosynthesis of the antituberculous agent caprazamycin
Taro Shiraishi, Prof. Makoto Nishiyama and Prof. Tomohisa Kuzuyama, Biotechnology Research Center, The University of Tokyo, Tokyo, Japan

Using shotgun metagenomics to investigate natural products made by symbiotic bacteria in marine sponges
Juan Lopera¹, Ian Miller¹, Theodore Weyna¹, Dr. Warren Rose² and Dr. Jason Kwan¹, (1)Division of Pharmaceutical Sciences, University of Wisconsin-Madison, Madison, WI, (2)Pharmacy Practice Division, University of Wisconsin-Madison, Madison, WI

Evaluation of the antimicrobial properties of formulated herb teas from the leaves and peels of five plants as functional food.
Chinwe Christy Isitua, Department of Biological Sciences, College of Sciences, Afe Babalola University Ado-Ekiti (ABUAD), Ado-Ekiti, Nigeria, Dr. Carmita Gladys Jaramillo Jaramillo, Planta Piloto de Farmacias, Facultad de Ciencias Quimicas y de la Salud, Universidad Tecnica de Machala, Machala, Ecuador, Ms. Diana Sanmartin Galvan, Microbiologia, Facultad de Ciencias Quimicas y de la Salud, Universidad Tecnica de Machala, Machala, Ecuador and Elizabeth Umoh, Department of Microbiology, Afe Babalola University, Ado-Ekiti, Nigeria

A novel inhibitor of Lactobacillus biofilms prevents stuck fermentations in a shake flask model
Lauren P. Saunders¹, Michael J. Bowman², Kenneth M. Bischoff¹ and Timothy D. Leathers¹, (1)Renewable Product Technology, United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, IL, (2)Bioenergy Research Unit, United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, IL

Engineering of new pneumocandin side-chain analogues by mutasynthesis and evaluation of their antifungal activity
Dr. Li Chen¹, Dr. Yan Li¹, Qun Yue¹, Dr. Anna Loksztejn², Prof. Kenichi Yokoyama², Mr. Edd Felix³, Prof. Xingzhong Liu⁴, Prof. Ningyan Zhang¹, Zhiqiang An¹ and Gerald F. Bills⁵, (1)Texas Therapeutics Institute, the Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, TX, (2)Department of Chemistry, Duke University, Durham, NC, (3)Pharmaceutical Science Facility, University of Texas MD Anderson Cancer Center, Houston, TX, (4)State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, (5)University of Texas Health Science Center at Houston, The Brown Foundation Institute of Molecular Medicine, Houston, TX

Production of combustible biogas using tomato crop residues 
Myrna Solís¹, Vanesa Chicatto¹, Aida Solis², Erik Ocaranza¹ and Lilia Tapia¹, (1)Centro de Investigacion en Biotecnologia Aplicada, INSTITUTO POLITECNICO NACIONAL, Tepetitla, Mexico, (2)UNIVERSIDAD AUTONOMA METROPOLITANA UNIDAD XOCHIMILCO, Ciudad de Mexico, Mexico

Aspergillus flavus secondary metabolism: one species, many compounds
Matthew Lebar¹, Dr. Jeffrey Cary¹, Brian Mack¹, Pamela Harris-Coward¹, Qijian Wei¹, Carol Carter-Wientjes¹, Deepak Bhatnagar¹, Dr. Jose Di Mavungu², Prof. Sarah DeSaeger² and Prof. Ana Calvo³, (1)USDA, New Orleans, LA, (2)University of Ghent, Gent, Belgium, (3)Northern Illinois University, DeKalb, IL

Biosynthesis of streptothricin and its related antibiotic BD-12
Haruka Niikura, Chitose Maruyama and Yoshimitsu Hamano, Department of Bioscience, Fukui Prefectural University, Fukui, Japan

Emestrins, anti-Cryptococcus epipolythiodiketopiperazines from Podospora australis
Yan Li¹, Qun Yue¹, Prof. James B. Gloer² and Gerald F. Bills¹, (1)Texas Therapeutics Institute, the Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, TX, (2)Department of Chemistry, University of Iowa, Iowa City, IA

Mechanisms of self-resistance in the echinocandin-producing fungus Pezicula radicicola NRRL12192
Qun Yue¹, Yan Li¹, Dr. Li Chen¹, Xiaoling Zhang², Jingzu Sun², Prof. Xingzhong Liu², Zhiqiang An¹ and Gerald F. Bills¹, (1)Texas Therapeutics Institute, the Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, TX, (2)State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

Establishment of a system for small molecule synthesis by Crude Lysates of Escherichia Coli
Jennifer Kay, Northwestern University, Evanston, IL