Insecticidal activity and repellency of rhamnolipids produced by Pseudomonas aeruginosa LBI 2A1 against the larvae of Aedes aegypti.

Aedes aegypti, is the mains vector of dengue, zyca viruses, yellow fever and chikungunya fever. This diseases are very important arboviruses just dengue affect about 100 millions of peoples per year. And causes around 30,000 deaths. Currently there is still no effective vaccine for dengue, zyca viruses and chikungunya fever, so the best way to control the diseases are control the vector population, that population control is done using insecticides, But insecticides can cause environmental damage, there are recent reports of insecticidal activity of rhamnolipids produced by Pseudomonas aerugionosa. This biosurfactant show low toxicity and is capable of reducing the interfacial and superficial tension. Rhamnolipids were produced in the bioreactor using mineral medium free of calcium and sunflower oil as a carbon source. The microbial strain used was Pseudomonas aeruginosa LBI 2A1. To evaluate the repellency were used 400, 800, and 1000mg/L of the rhamnolipds. A mice were sprayed with 2,400μL of the solutions. Each cage contained a mouse for each treatment and a total of 25 mosquitoes. For the insecticidal tests were used cages containing 20 mosquitoes and each one was prayed whit 3,200 μL of rhamnolipids solution. The host attraction attempts were reduced within creased concentrations of the biosurfactant. The mouses that was sprayed whit 1000mg/L were not bite. The insecticidal activity of rhamnolipids against mosquitoes showed a100% elimination of adults at the highest concentration evaluated compared to only 8% in the control group.