A Definitive Screening Design was employed to evaluate the cell growth and product concentration for an E. Coli strain under varying fermentation conditions. The experiments were conducted in 5L Sartoris A+ Bioreactors with controls for Temperature, pH, and DO levels. The fermentation process parameters evaluated with the definitive screening design were Temperature, pH, DO level, and Feed/Phosphate ratio. The responses measured for each experimental trial were OD600, Dry Cell Weight (DCW), and product concentration. The JMPâ statistical software (SAS Institute, Cary, NC) was used to analyze the experimental results and estimate predictive models for the responses. The models were subsequently used for optimization and sensitivity analysis to determine the relative impact of each experimental factor on the responses. The talk will discuss the analysis of results and conclusions drawn from the experiment. In addition a brief tutorial on the novel Definitive Screening Designs will be provided.
Cytovance Contributors: Samantha Head, Jesse McCool, April Stanley, Renija George, Jon Mitschelen, Anthony Hawrylecko, Stephen Strub, and Jobin Joy