Monday, August 13, 2012
Columbia Hall, Terrace Level (Washington Hilton)
There is the growing demand to develop highly efficient technology to produce ethanol and chemicals from woody biomass. For enzymatic conversion of lignocellulosic biomass, the pretreatment is necessary. Efficient conversion of the enzymatic hydrolyzates by ethanologenic microbes is also the key factor for ethanol production. To solve them, microwave-assisted solvolysis pretreatment has applied and novel high-performance ethanologenic bacteria are being bred through cell-surface engineering based on genome DNA analysis. In this study, ethanol production from pretreated eucalyptus wood chips using the recombinant xylose-utilizing Zymomonas mobilis [lvs::manA, pZA22-xtR] was carried out in a 30-L jar fermentor. As a result, over 50g/L ethanol was produced under the condition of 150g/L substrate loading.