Sunday, August 1, 2010
Pacific Concourse (Hyatt Regency San Francisco)
We demonstrated the capability of determining yeast concentration and viability in corn mash, where strong fluorescence signals from detected and counted from viable and nonviable yeast cells, while nonspecific staining of corn mash is minimized to facilitate effective automated cell counting algorithm. The development of the counting protocol employing Cellometer® Vision provides a simple tool for the biofuel industries to rapidly monitor yeast viability throughout a fermentation process to ensure optimal bioethanol output. Furthermore, this novel imaging cytometry method has the potential to simplify yeast research, which will be a tremendous impact on renewable energy development as fossil fuel reduction and global warming issues increase.