Sunday, August 1, 2010
Pacific Concourse (Hyatt Regency San Francisco)
In a study on the effect of biostimulation in bacterial diversity in soils contaminated by oil, was isolated the Dietzia cinnamea P4 strain belonging to the group Actinobacteria. This strain has shown to be excellent biodegrade of several n-alkanes and other derivatives of hydrocarbons linear and cyclical. This work aims to characterize the profile genome of P4 strain. The assembled genome contains 3,557,916 bp arranged in one single chromosome and its average G+C content is of 70,9%. P4 genome has complete pathways of all central metabolism pathways, also are present the glyoxalate and reductive carboxylate cycles. Autothrophic growth is suggested by the presence of aerobic CO-oxidizing genes, formate/formaldehyde oxidation, reverse TCA and 3-hydroproionate cycles for CO2 fixation. P4 has the genes for utilize very different sugars and chitin and cellulose compounds as sole carbon source. The secondary metabolism is present with genes for biosynthesis of terpenes compounds, frenolicin, nanaomycin and avilamycin A antibiotics, and a probably role in of Azinomycin B synthesis, a important product with antitumor actives. The complete Alk operon for degradation of alkanes is present, and clusters genes for biphenyl via ring dihydroxylation. This study brings news insights about the genetic and physiology of P4 strain, which could be useful in biotechnology and bioremediation applications.