P105: Production of xylanase and b-xylosidase by utilizing industrial biomass(sugarcane bagasse)

Hemicelluslose is the second abundant polysaccharide in nature after cellulose . Various enzymes which degrade hemicelluloses include xylanase and b-xylosidase. Microorganisms which produced the above mentioned enzyme are bacteria, yeast, fungi and actinomycetes. The present study was designed for the production of xylanases and b-xylosidase by Humicola lanuigosa  in solid state and submerged state fermentation were carried out by using sugar cane bagasse as a substrate. Enzyme production and substrate utilization by Humicola lanuigosaus  were maximum in solid state fermentation than that in submerged fermentation. The kinetic study revealed that the volumetric rate of xylanase and b-xylosidase production (Qp, IU/I/h) was 6.55 and 0.41 respectively through solid state fermenation. While Product yield coefficient of xylanase and b-xylosidase namely Yp/x and Yp/s were 331.43 IU/gs , 1902.17 IU/g cell and 32.91IU/gs, 188.9 IU/g cell respectively.