Otman Ermithi Abdul bast .R.Abuzweda Mhamed Ali Hasen
ermithi@yahoo.com
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Molasses is the most important element of yeast industry, and the countries that are suffering from shortage of this substance is largely focused on the search for alternatives that could be used in this industry and among which was concentrated juice of dates, Hence, the starting point for this research study so as to determine the appropriate of concentrated juice of dates for the growth of 5 isolates of yeast (Saccharomyces cervisia) locally isolated from wheat, barley ,date and from Tripoli Libya.
The study starts by preparing different concentrations of the juice of dates (25, 50, 75 and 100%) with barley extract was distributed in equal amounts (100 ml) in conical flasks of 250 ml capacity in triplicates, after that a 1 ml of Dextrose yeast extract peptone broth containing the concentration of approximately 1 X 106 cfu\ml of Saccharomyces cervisia of the 5 isolates and commercial yeast ( pakmay ) as control were added and incubated in shaker incubator at 30oC for 48/hrs
To find out the activity of yeast the dilution method was used to determine the total count, the results showed that the best concentration for yeast cultivation was 25% and followed by 50% and 75% there were clear differences between them but there no clear different 75% and 100% concentration .The best cultivation was recognized with the barley isolates at 25%.
P ≤ 1%