P23: Bioinformatics analysis of fungi genome

Sunday, August 1, 2010
Pacific Concourse (Hyatt Regency San Francisco)
Dongfang Li1, Nan Qin2 and Jun Wang2, (1)Bioinfromatics center, BGI(formly known as Beijing Genomics Institute) and Medical School of Shenzhen University, Shenzhen, China, (2)Microbial Genomics, BGI(formly known as Beijing Genomics Institute), Shenzhen, China
With the breakthrough of the new-generation sequencing technology which results in lower costs, higher throughout and superior accuracy, we can obtain more and better informations about the fungi genomes.

BGI has completed and is undertaking some projects about fungi such as Saccharomyces cerevisiae, Trichoder makoningii, Netarhizium anisopliae, Verticillium dahliae Kleb, Fusarium oxysporium et.al. Generally, there are two analysis ways for fungi genome:de novo analysis and re-sequencing analysis. The main contents are showed as follow, respectively.

De novo analysis: De novo sequencing , Assembly, Annotation,

Re-sequencing analysis: Sequencing, Sequencing depth distribution and genome coverage calculation, Consensus sequences assembly according to the reference genome.

If related species were given in the de novo analysis, both ways could perform the genome comparison to identify structure variation and the homologous relationships with the references genome, and we also do the SNP and Indel calling, segmental duplication, rearrangement with the references, and gene evolution and selection, et.al.