Sunday, August 1, 2010
Pacific Concourse (Hyatt Regency San Francisco)
In the past, 10 mL disposable bioreactors in arrays of twenty-four vessels (Micro-24 Micro Bioreactor) were limited to microbial applications; while the options for the cell culture community remained uncontrolled vessels (Plates, T-flasks, and Shake Flasks) or larger scale bioreactors, which are resource intensive and often limited in number (very low experimental throughput). Today, 10 mL reactors are being used successfully to not only screen cell lines in a controlled environment as part of cell line selection, but also to screen culture conditions (media development, feed development, set point optimization) as part of process optimization, DoE studies, and QbD initiatives. The goal is to increase the likelihood of obtaining commercial cell lines as well as defining the biotech production processes earlier in development. This poster will present the evolution of applications from microbial cultures to CHO using the Micro-24 system.