P7: Production of Thermostable Lipase Isolated from Fungi

250 soil samples were collected from different location in Saudi Arabia. 136 fungi isolates were identified by microbiological, biochemical and molecular technique. Ribosomal DNA (rDNA) and some other gene sequences have been successfully used to reveal the phylogenetic relationships within the fungi orders. The distribution of different lipolytic fungi was able to be identified in various environmental samples. Most of the fungal species gave low production after 24 hr of incubation. It was also noticed that there was an increase in the activity at 48 and 72 hrs in nearly most of the strains. For various carbon sources, results indicate that the highest activity detected was in Tween 20 followed by olive oil and Tween 40, Tween 60 and Tween 80.