Identification and bio-prospective effects of novel quinone molecule from Aegle marmelos (Linn.) Correa against pathogenic bacteria and filamentous fungi

The present study focused on identification and characterization of novel bio-molecule from Aegle marmelos (Linn.) Correa and its activity against pathogenic bacteria and filamentous fungi. The antimicrobial activity of the crude organic solvent extract by disc diffusion method exhibited good activity against Gram positive and Gram negative bacteria. A compound was obtained by eluting the crude extract using varying concentrations of solvents followed by the chromatographic purification by HPLC. Based on the MS, IR, ¹³C NMR and ¹H NMR spectral data, the compound was identified as quinone related antibiotic. It exhibited significant activity against Gram positive and Gram negative bacteria. The lowest Minimum Inhibitory Concentration (MIC) of the compound against Bacillus subtilis and Staphylococcus aureus were 100 and 75 µg mL^-1 respectively. Against Escherichia coli and Pseudomonas aeruginosa it exhibited MIC value of 25 µg mL^-1. The MIC of the compound against Aspergillus clavatus, A. niger, Curvaleria lunata, G. moniliformis, and P. roqueforti was 20 µg mL^-1 and that against C. acuminatus, H. grisea, P. chrysogenum, S. vaccinii and S. brevicaulis, was 100 µg mL^-1, respectively. The results concluded that the compound can be evaluated further in industrial applications and also an agent to prepare bio-control formulations.