P7: A New Generation of ATP Bioluminesence Methods for Rapid Analysis of Microorganisms in Industrial Products

Advances in technology have lead to the development of a number of rapid microbiological monitoring methods over the last few decades, including methods based on the detection of ATP (adenosine triphosphate). Historically, ATP-based methods were susceptible to interferences caused by various components in a sample. Consequently, traditional ATP test methods have not been sustainably applied in wastewater and industrial applications.

Developments in the reagents and methodology used have led to substantial improvements in the ATP test method. The authors have developed a portfolio of next-generation ATP test methods designed specifically for fluid samples, which are typically laden with interferences. These new protocols and reagents were designed to minimize and neutralize various types of interferences including suspended solids, dissolved solids, biocides and organics. This is accomplished using a combination of separation and chemical treatment to isolate microorganisms from interference. This presentation will present the results of validation tests performed on various complex samples including latex emulsions, mineral slurries, concrete admixtures, personal care products and fuels.