S4: Cell Line Development Tool Box for Expression: E.coli, Pichia, CHO, Insect Cells

Title: Cell Line Development Tool Box for Expression: E.coli, Pichia, CHO, Insect Cells Sam Ellis                                  Department of Biotechnology, Thomson Instrument Company                                      1121 S. Cleveland St. Oceanside Ca. 92054                                                   folks@htslabs.com , PH: +760-757-8080                                               Cell Line development: The conditions for E.coli, Pichia, CHO and Insect Cell lines need to be maintained at small scale and within fermentation. Data will be presented on techniques and technology that allow for mimicking large scale fermentation with non-controlled devices from 1mL-2.5L. All of these techniques are proven technologies for protein production, structural biology, and can lead to successful transfer from cell line development to bioprocess group. Keywords:  Cell Line Development, E.coli, Pichia, fermentation, CHO, Hek293.