Monday, November 9, 2009
P38
USE OF PAPER MILL RESIDUES FOR THE SECOND GENERATION ETHANOL PRODUCTION BY SIMULTANEOUS SACCHARIFICATION FERMENTATION PROCESS
Edelvio B. Gomes, Eleandro M. Walverde, Elton F. Nascimento Jr, Daiana S. Neves, Luiz A. F. S. Schilittler, Luiz C. S. Carlos, and Nei Pereira Jr. Biochemical Engineering, Federal University of Rio de Janeiro, Avenida Athos da Silveira Ramos, Rio de Janeiro, Brazil
The aim of this work was to investigate the potential of ethanol production from paper mill residues. Rejected wood chips were used as biomass. Wood chips were pretreated with sulfuric acid (1.5 % v / v; 121 ºC; 40 minutes). The solid fraction was separated using three delignification conditions: recovered alkaline liquor (at 10; 40 and 124.8g/L); NaOH (40g / L) and cooking liquor. Enzyme concentration (Multifact - Novozymes A/S) was adjusted to 10 FPU / g of biomass. Enzymatic hydrolysis was executed previously by 12 hours (50 ºC; 200 rpm). Afterwards, the system was inoculated with the yeast strain of Saccharomyces cerevisiae to starts the ethanol production by simultaneous saccharification and fermentation process (SSF). A central composite statistical experimental design was applied in order to investigate the optimum of enzymatic concentration and solid / liquid ratio. Production of glucose from the pre-hydrolysis was greater in the treated biomass with 40g / L of the alkaline liquor, achieving 17.8g / L. The highest production of ethanol (6.7 g / L) was observed to the biomass treated with 10g / L after 33-hours of SSF. Experiments indicated optimum of enzyme concentration of 30 FPU / g of biomass. Results showed the potential of utilization of the rejected wood chips as a raw material to produce ethanol by means of the reuse of the diluted alkaline liquor.
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