Monday, November 9, 2009
P35
Evaluating strains in millilitre scale cultivations including experiences with the µ24 MicroBioreactor from Applikon/Micro Reactor Technologies
Søren Michaelsen, Microbial Physiology, Expression Technology, R&D, Novozymes A/S, Krogshøjvej 36, Bagsværd, 2880, Denmark
A large number of strains are constructed when new products, variants or optimised strains are made. Primary evaluation of strains has typically been done in shake flasks, whereas secondary screening of the selected best ones in 1,5 L bioreactors. However, comparison of results between shake flasks and bioreactors has often shown no or poor correlation of the ranking of strains in the two cultivation setups. Often, the number of lab bioreactors is limited, and running bioreactors is resource demanding. Therefore, we wanted a small scale cultivation evaluation of strains that correlated well with bioreactors. The cultivation equipment used for this should preferably be able to feed in carbohydrate, and with the ideal setup the most important cultivation parameters should be measured and controlled. Initially fed batch shake flasks were tested with peristaltic pumps for feeding and slow release shake flasks were also tested. Furthermore, the µ24 MicroBioreactor from Applikon/Micro Reactor Technologies was evaluated. Our experiences with the different cultivation systems along with obtained cultivation results will be shown.
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