Disposable bioreactors have been incorporated into clinical- and production-scale biotechnological facilities at an increasing rate over the past few years. The driver for the development and popularity of disposable bioreactors in cell culture processes is the Wave Bioreactor System 20, which was first used in 1998. In this bioreactor wave-facilitating mixing and oxygenation is induced in a flexible bag (containing culture medium and cells) by one-dimensional rocking of its tempered platform. However, in order to understand the mixing principle and to allow comparison with other bioreactor types, it was necessary to investigate the engineering parameters of wave-mixed bioreactors. Computational Fluid Dynamics (CFD) is a suitable tool for predicting fluid flow as well as local- and time-dependent gradients of temperature, pressure, velocity, shear stress etc. Simulations for the single use Wave bag (2L) were accomplished with commercial CFD code. The Volume-of-Fluid model (VOF) and the sst-kΩ turbulence model were used to calculate free surface of the liquid and fluid flow. The 3D simulations suggest power input of a theoretically reasonable magnitude. The water-air interface formation of the model corresponds to video recordings previously made. Results of previous investigations of mixing time and mass transfer rates will be given. Additionally, an overview of further experimental investigations (including high speed shots and measurements of power input and fluid velocity) will be included to verify simulation results. In addition to engineering characterization, cell culture processes with Chinese hamster ovary (CHO) cells, insect cells (SF9) and plant cells will be compared with simulation results.