Monday, November 9, 2009 - 8:00 AM
S7
Disposable continuous culture microbioreactor with yeast for screening and process development applications
Anna Eliasson Lantz1, Daniel Schäpper2, Stuart M. Stocks3, Nicolas Szita4, and Krist V. Gernaey2. (1) Department of Systems Biology, Technical University of Denmark, 2800 Lyngby, Denmark, (2) Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800 Lyngby, Denmark, (3) Novozymes, DK-2880 Bagsvaerd, Denmark, (4) Department of Biochemical Engineering, University College London, London, WC1E 7JE, United Kingdom
In the research and development phase of industrial fermentation processes, typically a tremendous research effort is put into both optimisation of the strain and the process conditions. This is often done in microtiter plates or by using shake-flask cultures as they are easy to set up. However, only batch experiments are possible and conditions are not well-controlled. This might result in deviations from results that would be obtained in industrial scale reactors. Bench-scale reactor cultivations provide an alternative where conditions can be well-controlled and industrial conditions mimicked. However, this type of cultivations are rather labour intense and are therefore not a viable alternative when large number of strains or conditions should be screened.
Microbioreactors offer the possibility to circumvent many of the above-mentioned problems: (1) Many parallel reactors allows for high-throughput screening; (2) The working volumes are very small, keeping costs low; (3) On-line measurements and control are possible (4) The reactors can be fabricated from polymers, thus making them disposable after use; (5) Finally, for batch type microbioreactors, the fermentation variables compare favourably with bench scale reactors, which indicates that the right culture physiology can be maintained at small vs. larger scale, making the technology commercially viable.
This work reports on the development of a continuous culture microbioreactor platform. A membrane-aerated, polymer-based microbioreactor that has integrated mixing and heating and allows for on-line measurement of OD, DO and pH is presented. Design considerations and results of S. cerevisiae cultivations performed in the microbioreactor will be reported and compared to literature data.
Microbioreactors offer the possibility to circumvent many of the above-mentioned problems: (1) Many parallel reactors allows for high-throughput screening; (2) The working volumes are very small, keeping costs low; (3) On-line measurements and control are possible (4) The reactors can be fabricated from polymers, thus making them disposable after use; (5) Finally, for batch type microbioreactors, the fermentation variables compare favourably with bench scale reactors, which indicates that the right culture physiology can be maintained at small vs. larger scale, making the technology commercially viable.
This work reports on the development of a continuous culture microbioreactor platform. A membrane-aerated, polymer-based microbioreactor that has integrated mixing and heating and allows for on-line measurement of OD, DO and pH is presented. Design considerations and results of S. cerevisiae cultivations performed in the microbioreactor will be reported and compared to literature data.
See more of Microbial culture scale up, manufacturing, and process characterization
See more of Invited Oral Papers
See more of RAFT VIII (November 8 - 11, 2009)
See more of Invited Oral Papers
See more of RAFT VIII (November 8 - 11, 2009)