Monday, November 9, 2009
P9

BioIsopreneTM: Development of a bio-based process for production of isoprene from renewable resources

Ilana S. Aldor1, Anthony R. Calabria1, and Joseph C. McAuliffe2. (1) Microbial Physiology and Fermentation, Genencor, 925 Page Mill Road, Palo Alto, CA 94304, (2) Biochemistry, Genencor, 925 Page Mill Road, Palo Alto, CA 94304

Genencor, a Danisco Division, is collaborating with The Goodyear Tire & Rubber Company to make BioIsopreneTM monomer produced via microbial fermentation. The BioIsopreneTM product is in the gas phase, and in situ purification from the fermentation off-gas relieves end-product inhibition and toxicity within the fermentor. Our strain generation efforts produce too many metabolically engineered microbes to run in our 14-L fermentation facility in a cost-effective manner. Therefore, we use 96-well plates, shake flasks and a 24-well CelleratorTM as scale-down models to screen strains. The latter instrument has independent control of pH, temperature and dissolved oxygen in each well. Our work has enabled us to produce the equivalent of over 60 g/L monomer in the fermentation volume.

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