Monday, November 9, 2009
P6
Ambient gas exchange enabled stirred culture bottle -- a S.T.R. version of shake flask for high gas-liquid mass transfer
Duen G. Mou, Moubio Inc., 10F-2, 90 Jo Kon St., Taipei, 00116, Taiwan
Through gas vent riser tube or gas intake downer tube, spent gas exhaust and ambient substrate gas intake in a stirred bottle culture are an order of magnitude enhanced without line gas sparging when compared to similar size shake flask culture. In addition to realistic mixing, the novel in-situ ambient gas exchange design, tested in a 500ml wide-mouth bottle, produced an E. coli growth at 90% working volume equal or better than that of a reciprocating 250ml Erlenmeyer flask at 20% through increased gas ventilation and gas hold-up. Gas intake downer tube version also produced comparable gas transfer capacity at much reduced agitation power and impeller shear. This new culture bottle bridges the oxygenation gap between flask shaker and jar fermentor with added benefit in small footprint, low power usage and personalized convenience. (International patents and pending application)
1. 1100rpm 38mm 6-blade paddle turbine
2. 400rpm 46mm 6-blade Rushton turbine
3. increments between 3-5hr, period with steepest linear growth
4. oxygen uptake rate (OUR) is calculated by converting ΔOD to oxygen consumption
5. oxygen transfer capacity (OTC) is oxygen transferred in an hour to each respective vessel
Table I Oxygen uptake rate in and oxygen transfer capacity of various culture vessels under different aeration condition measured by growing 37oC E. coli in Luria Broth medium
| Reactor type | Batch volume, ml | Working volume, % | ΔOD3 absorbance unit | OUR4 mmol/L-hr | OTC5 mmol/hr |
| 500ml stirred bottle1 with air riser tube | 450 | 90 | 0.86 | 7.7 | 3.4 |
| 500ml stirred bottle2 with air downer tube | 450 | 90 | 0.74 | 6.6 | 3.0 |
| 250ml flask, 50 mm/220 rpm rotary shaker | 50 | 20 | 0.55 | 4.9 | 0.25 |
| 250ml flask, 50 mm/120 spm reciprocating shaker | 50 | 20 | 0.57 | 5.1 | 0.25 |
| 250ml flask, 50 mm/120 spm reciprocating shaker | 25 | 10 | 0.80 | 7.2 | 0.17 |
2. 400rpm 46mm 6-blade Rushton turbine
3. increments between 3-5hr, period with steepest linear growth
4. oxygen uptake rate (OUR) is calculated by converting ΔOD to oxygen consumption
5. oxygen transfer capacity (OTC) is oxygen transferred in an hour to each respective vessel
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