Monday, November 9, 2009
P24
ADVANCES IN USING CAPACITANCE BASED BIOMASS PROBES IN DISPOSABLE BIOREACTORS AND cGMP
With the increasing trend toward using disposable bioreactors, there is now a need for a range of viable biomass probes suitable for these systems, especially if the disposable bioreactors are being used in process development or for the production of high cell densities. A single-use biomass probe will also eliminate the risk of introducing contamination into a sterile gamma irradiated bag from either frequent sampling or introducing a traditional reusable probe.
In the present study we describe in detail for a low cost, single-use probe for use with the capacitance based Biomass Monitor (Aber Instruments,UK). We show how both the single-use and reusable biomass probes produce the same radio-frequency field allowing users to directly compare viable cell density data between conventional and disposable bioreactors.
In order to demonstrate the performance of the disposable biomass probes, data will be presented on the growth of animal cells in disposable bioreactors. The on-line data from the disposable biomass probe will be compared to the off-line data using traditional cell counting methods. We also show how modified reusable biomass probes can be aseptically introduced into the larger disposable bioreactors by utilizing the Kleenpak Pall or equivalent interface. On-line viable cell concentration data from a Hyclone SUB using a reusable biomass probe is compared to off-line results.
The study shows that the addition of biomass probes in both reusable and single-use formats to disposable bags will greatly enhance the opportunity to use these bioreactors in both process development and ultimately in cGMP manufacturing
In the present study we describe in detail for a low cost, single-use probe for use with the capacitance based Biomass Monitor (Aber Instruments,UK). We show how both the single-use and reusable biomass probes produce the same radio-frequency field allowing users to directly compare viable cell density data between conventional and disposable bioreactors.
In order to demonstrate the performance of the disposable biomass probes, data will be presented on the growth of animal cells in disposable bioreactors. The on-line data from the disposable biomass probe will be compared to the off-line data using traditional cell counting methods. We also show how modified reusable biomass probes can be aseptically introduced into the larger disposable bioreactors by utilizing the Kleenpak Pall or equivalent interface. On-line viable cell concentration data from a Hyclone SUB using a reusable biomass probe is compared to off-line results.
The study shows that the addition of biomass probes in both reusable and single-use formats to disposable bags will greatly enhance the opportunity to use these bioreactors in both process development and ultimately in cGMP manufacturing
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