Disposable fermentation technologies can provide significant cost efficiencies and flexibility at the manufacturing scale.  At small scales, ease of set-up can lead to more efficient process development, allowing execution of DoE to define the multivariate design space of the fermentation process in a parallel manner.  The question still remains as to how disposable technologies at these two extreme scales can be integrated to ensure efficient process development that is transferable to the manufacturing scale.  Case studies of rapid simultaneous production strain selection and development of a high productivity fermentation process using a disposable minibioreactor and its subsequent successful scale-up to a disposable production bioreactor will be presented.