S33: Maximizing Product Quality and Production through Biochemical Profiling to Enhance Cell Factory Performance

Maximizing the production of biomolecules and biofuels in cell culture and fermentation systems remains empirical and requires multiple rounds of systematic analysis to resolve bioprocessing issues. Biochemical profiling provides extensive systems information to facilitate critical choices in strain design and selection, process development and scale up for improved system productivity. Biochemical changes can be correlated to performance data (e.g. production over time, cell viability, pH) and profiling of raw materials such as base and feed media components can identify differences which can be correlated to bioreactor performance. A global metabolic approach surveys the maximum number of biochemicals (metabolites) and identifies those affecting cell metabolism at all stages of bioprocess development and production in bacteria, yeast, algae, plant and mammalian cell expression systems. Case studies demonstrate how changes in the biochemical profile have been studied in these systems and were effectively leveraged in bioproduction and fermentation systems for improved production and product quality.