Monday, November 4, 2013
Capri Ballroom (Marriott Marco Island)
Growth media for Saccharomyces cerevisiae (S. cerevisiae ) either supports faster growth with lower final biomass, or slower growth with higher final biomass. The goal of this study was to develop a peptone for S. cerevisiae that provides optimum growth throughout the growth cycle. Thirteen Sensient peptones were screened for S. cerevisiae growth by shake flask method. Five of the best performing peptones were chosen for further analysis to identify key peptone properties that affect the growth of S. cerevisiae. Analysis of total protein content of each peptone with growth did not reveal any correlation. Therefore, protein content was further analyzed by peptide molecular weight (PMW) profile as determined by size exclusion chromatography using HPLC. The percentage of peptides in high, medium, and low molecular weight range were calculated for each peptone. S. cerevisiae growth, both at 8 and 24 hours, was found to correlate with percentage of high molecular weight fraction. Correlation was negative at 8 hour and positive at 24 hour time points. Hence, optimized peptide molecular weight profile of a peptone can provide best overall growth. New peptones were developed based on this theory. The growth studies using the new peptones confirm that optimized peptide molecular weight profile provides enhanced growth of S. cerevisiae.