Cyanobacteria have numerous advantages over terrestrial crops as a platform for production of renewable sugars for fuels, chemicals, and biomaterials. They can be cultivated in salt water, do not require arable land, are capable of nitrogen fixation, and secrete exopolysaccharides directly into cultivation medium. As part of collaboration between Heliobiosys Inc. and Lawrence Berkeley National Lab, a consortium of wild type, nitrogen-fixing, marine cyanobacteria was cultivated at 20L scale. These cultures reached a total chemical oxygen demand (COD) exceeding 1.5g/L in the cultivation medium, with polysaccharides and other excreted products accounting for over half of the total COD. Polysaccharides in the cultivation medium were concentrated, de-salted, and precipitated via a scalable membrane filtration-based process. The pure form polysaccharides have biomaterial applications and readily form hydrated gels containing 0.1-0.5% solids by dry weight. Characterization of the polysaccharides following NREL LAPS revealed a mixture of 5 and 6-carbon sugars including significant fractions of glucose, xylose, arabinose, galactose, and mannose, alongside a significant fraction of unidentified sugars. Analytical method development to characterize the remainder of the cyanobacterial sugars is ongoing. Hydrolyzed polysaccharide was fermented with Rhodosporidium toruloides, establishing performance comparable to that of a pure glucose control. These results confirm the viability of cyanobacterial sugars as a potential low-cost fermentation feedstock. We are now pursuing pilot-scale testing of cultivation and recovery systems in 3000L raceway ponds. Scale-up work will focus on maximizing production rates, recovering and testing polysaccharides for downstream applications, and evaluating process economics under optimized conditions.