M128 Accessory enzymes produced by a newly isolated Lasiodiplodia theobromae from Amazon forest with potential for use in biomass degradation
Monday, April 27, 2015
Aventine Ballroom ABC/Grand Foyer, Ballroom Level
Carolina Brás Costa1, Priscila da Silva Delabona2, Gisele Nunes Rodrigues3, Daniel Kolling4, Patricia dos Santos Costa5, Deise Juliana da Silva Lima5, Marcia da Silva Souza6, Marcela Oliveira Lee6, Alberdan Silva Santos6 and José Geraldo da Cruz Pradella7, (1)Brazilian Laboratory of Science and Technology of Bioethanol - CTBE, Campinas, Brazil, (2)Brazilian Laboratory of Science and Technology of Bioethanol - CTBE, Campinas, Brazil, Campinas, Brazil, (3)Brazilian Laboratory of Science and Technology of Bioethanol - CTBE, Brazil, Campinas, (4)Brazilian Laboratory of Science and Technology of Bioethanol - CTBE, Brazil, (5)Brazilian Laboratory of Science and Technology of Bioethanol - CTBE, Campinas, Brazil, Brazil, (6)Universidade Federal do Pará, Belém, (7)Brazilian Laboratory of Science and Technology of Bioethanol - CTBE, Brazil, Campinas, Brazil
Bioethanol from cellulosic biomass is one of the most promising biofuels to lessen the environmental pollution generated by burning of fossil fuels. Accessory enzymes that assist biomass degradation could be used to improve the recovery of fermentable
sugar for use in biorefineries. Therefore, optimization studies of enzyme complex to obtain high sugar yield have been done. Synergism between enzymes may play the major role to obtain a high sugar yield. Delabona et al. 2013 suggested important roles for pectinase and α-L-arabinofuranosidase in the enzymatic synergies inherent in sugarcane bagasse hydrolysis, making the process more effective and competitive. In this study, different fungal strains isolated from the Amazon rainforest were evaluated in terms of their ability to produce cellulases, xylanses, pectinases and α-L-arabinofuranosidase enzymes. In the selection step, screening using plate assays was followed by shake flask submerged cultivations using Celufloc™ and pretreated sugarcane bagasse. A new strain of Lasiodiplodia theobromae MIBA-0016 was found to be a potential candidate for the formulation of optimized enzyme mixtures for the saccharification of plant biomass. Before moving on to study the strains in a larger-scale bioreactor, it was decided to modulate enzymatic activities relevant for the hydrolysis biomass using different carbon sources (yeast extract, soybean bran, wheat bran, sucrose and orange peel) on enzymes production by fractional factorial designs. Significant increases were obtained for β-glucosidase activity (120%) and pectinase (105%) and theses enzymes were used for hydrolysis biomass resulting in greater sugar release