M130
Evaluation of chemical treatment for glucose production from steam exploded reed (Phragmites australis)
Monday, April 27, 2015
Aventine Ballroom ABC/Grand Foyer, Ballroom Level
Reed (Phragmites australis) is often recognized as a promising source of renewable energy. In the present study, the combined pretreatment of reed using steam explosion and chemical (1% NaOH, 1% H2O2 and 95% methanol) and its delignification rate and enzymatic hydrolysis were investigated.
The steam explosion was performed under conditions of low severity to optimize the amount of solid recovery. Reed was submitted to steam explosion at severity log Ro 4.03.
In the steam exploded reed was impregnated again with chemical (1% NaOH, 1% H2O2 and 95% methanol) to remove a portion of the lignin, and to make the cellulose more accessible to enzymatic attack. The steam exploded (severity log Ro 4.03)-chemical treated reed showed delignification rate and high glucose conversion. The delignification rate was 5.5%, 77.3% and 19.1% for the steam exploded-1% NaOH treated reed, steam exploded-1% H2O2 treated reed and steam exploded-methanol treated reed, respectively. Steam exploded-1% H2O2 treated reed was converted to 24.5 g (based on 24.9 g cellulose) glucose with 98.4% yield efficiency.
The enzymatic hydrolysate of steam exploded-1% H2O2 treated reed was converted into ethanol efficiently by Saccharomyces cerevisiae KCTC 7296 and the ethanol yield from glucose (based on 24.5 g glucose) was about 64% (w/w) of the theoretical value. These results indicate that 1% H2O2 treatment increased enzymatic digestibility and ethanol productivity from steam exploded reed. The research results are meaningful in bioconversion and utilization of renewable lignocellulosic biomass.
The steam explosion was performed under conditions of low severity to optimize the amount of solid recovery. Reed was submitted to steam explosion at severity log Ro 4.03.
In the steam exploded reed was impregnated again with chemical (1% NaOH, 1% H2O2 and 95% methanol) to remove a portion of the lignin, and to make the cellulose more accessible to enzymatic attack. The steam exploded (severity log Ro 4.03)-chemical treated reed showed delignification rate and high glucose conversion. The delignification rate was 5.5%, 77.3% and 19.1% for the steam exploded-1% NaOH treated reed, steam exploded-1% H2O2 treated reed and steam exploded-methanol treated reed, respectively. Steam exploded-1% H2O2 treated reed was converted to 24.5 g (based on 24.9 g cellulose) glucose with 98.4% yield efficiency.
The enzymatic hydrolysate of steam exploded-1% H2O2 treated reed was converted into ethanol efficiently by Saccharomyces cerevisiae KCTC 7296 and the ethanol yield from glucose (based on 24.5 g glucose) was about 64% (w/w) of the theoretical value. These results indicate that 1% H2O2 treatment increased enzymatic digestibility and ethanol productivity from steam exploded reed. The research results are meaningful in bioconversion and utilization of renewable lignocellulosic biomass.