High cell density fermentation of Escherichia coli using the Eppendorf BioFlo® 320 bioprocess control station

Escherichia coli (E. coli) is a Gram-negative bacterium that has had a long history in the world of laboratory and industrial processes due to its ease of manipulation and well understood genome. It is widely cultured under aerobic conditions. High cell density cultivation of E. coli is a powerful technique for the production of recombinant proteins. Indeed, 30 % of the FDA-approved biopharmaceuticals on the market are produced in E. coli.

In this poster, an E. coli fermentation run was conducted using the new Eppendorf BioFlo 320 bioprocess control station, which is an advanced bioprocess system developed for both microbial fermentation and cell culture applications. High cell density was achieved at 12 h as determined by a maximum optical density (OD₆₀₀) measurement of 215.2. The wet cell weight (WCW), dry cell weight (DCW), and volumetric oxygen transfer coefficient (k_La) were also measured and presented.