Transcriptomic analysis of Aspergillus terreus grown on agro-industrial residues
Tuesday, April 29, 2014
Exhibit/Poster Hall, lower level (Hilton Clearwater Beach)
C. L Corrêa, E.F. Noronha, E.F.F. Ximenes and R.N.G. Miller, Departamento de Biologia Celular, Universidade de Brasília, Brasília, Brazil
Increased demands in global energy consumption, together with concerns over environmental pollution are driving the development of energy sources to meet future energy demands. Aspergillus terreus is a filamentous fungus producing enzymes with a wide range of biotechnological applications. This study aims to characterize lignocellulolytic enzyme production in A. terreus grown on culture media containing agro-industrial residue (sugarcane bagasse, soybean hull and louse cotton) and analyze differential gene expression in response to growth sources. A. terreus grown at 28°C for each carbon source (1% ) over a ten day period produced enzymatic activities of xylanase, endoglucanase, mannanase, pectinase and FPase identified according to the DNS method. Whilst xylanase enzyme activity reached a plateau at 48 hours for all three carbon sources, production of this enzyme was more significant for the media containing sugarcane bagasse and soybean hulls, reaching 1.0 (IU.ml-1). The production of pectinase and FPase was more prominent in the medium containing soybean hulls, reaching 0.5 and 2.0 (IU.ml-1), respectively. cDNA library preparation for fungal mRNA from A. terreus cultured was conducted using the RNA TruSeq Sample preparation v2 Kit (® Illumina, Inc.). A. terreus also showed a significant performance as a producer of holocellulases. The sequenced cDNA libraries yielded a total average of 2.7 GB, with approximately 27 million reads. Approximately 81% of reads displayed a quality of Q >=30). Characterization of these transcriptome offers promise gene discovery for application in second generation biofuel development.