Production of Holocellulases and Amylases by filamentous fungi grown on alternative substrates
Monday, April 28, 2014
Exhibit/Poster Hall, lower level (Hilton Clearwater Beach)
Bruna dos S. Menezes1, Katia dos S. Morais1, Tulio C. R. Leite1, Lucas M. Alcantara2, Edivaldo X. Ferreira Filho3, Bruno dos S. A. F. Brasil4 and Felix G. de Siqueira5, (1)Laboratório de Enzimologia e Microbiologia Industrial - LEMI, Universidade Federal da Bahia - Campus Anísio Teixeira, Vitoria da Conquista/BA, Brazil, (2)Departamento de Biotecnologia, Universidade Federal da Bahia, Vitoria da Conquista, Brazil, (3)Cellular Biology, University of Brasília, Brasília, Brazil, (4)Laboratory of Biochemical Processes, Embrapa, Brasilia/DF, Brazil, (5)Laboratory of Biochemical Processes, Embrapa Agoenergia, Brasilia/DF, Brazil
The Brazil semiarid is a region with low rainfall, however, rich in biodiversity under explored, but with great potential to be used as substrate in fermentation processes, as they have good reserves of water and carbohydrates. In this work, enzyme preparations of three fungi isolated were produced by growing it in submerged culture medium (1.0% w/v) and semi-solid medium,  with C-source: cassava (Manihot esculenta), Jacaratia corumbensis and Mirabilis jalapa, plants that are commonly found in Brazil semiarid Northeastern. Enzyme activities (xylanase, pectinase, mannanase, FPAtotal, CMCase, Avicelase and amylase) were characterized. Subsequently, selected the best fungus grown on biomass for enzymatic characterization of xylanase and CMCase by column chromatography (G-50). The products were checked on denaturing SDS-PAGE 10% gel for separation and identification of enzymes. It is noteworthy, especially for xylanase activity (1.2 UI.mL-1) and CMCase (0.23 UI.mL-1), where the isolated VCMR15 was cultured in J. corumbensis. However, the xylanase activity (0.4 UI.mL-1) and CMCase (0.2 UI.mL-1) for cassava cultivation in liquid proved relevant. Some activities were observed significant mannanase, pectinase and amylase in semisolid culture medium. However, the same was not observed in submerged cultivation. Possibly separate xylanase was isolated from VCMR15 grown under water having as C-source J.   corumbensis after column chromatography. The microbial enzymes have great potential for application in industry and in the search for new substrates with low cost are important. However, more studies are needed, with the possibility of pre-treatment to see if there will be better enzymatic activities and characterization of enzymes.