Extraction and characterization of essential oil from pine needles for potential antimicrobial applications
Tuesday, April 29, 2014
Exhibit/Poster Hall, lower level (Hilton Clearwater Beach)
Elizabeth Martin1, Steven C. Ricke2, Kristen Gibson2, Giselle Almeida2, Danielle J. Carrier1 and Joshua Adams3, (1)Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR, (2)Food Science, University of Arkansas, Fayetteville, AR, (3)School of Forest Resources, University of Arkansas, Monticello, Monticello, AR
Staphylococcus aureus is the causative agent for numerous human infections, including superficial skin infections, and more serious deep abscesses. Essential oils or fractions of oils from pine needles were proposed as a topical antimicrobial agent against serious strains of methicillin resistant S. aureus (MRSA). Pine needle oil was extracted from Loblolly pine needles (Pinus taeda L.) by water distillation using a Clevenger apparatus. By GC/MS analysis, it was determined that the major oils were α pinene (0.3-2.0 mg/g), β pinene (0.1-1.78 mg/g), limonene (0.01-2.1 mg/g) and terpineol (0.01-2.1 mg/g). Essential oils from pine are reported to have antimicrobial effects against pathogenic bacteria, including S. aureus. The extracted oils from this research are being analyzed for antimicrobial activity using disc diffusion and other analytical assays.