The results of these processes at different conditions of time, temperature and reagents concentration yielded 82% as the maximum concentration of cellulose for organosolv pretreatment (190ºC, 90 min and 50% v/v). At this condition, the lignin and hemicellulose removal were estimated as 78.3 % and 86.8 %. However, the highest conversion in enzymatic hydrolysis (88.2%) was observed with lower levels of lignin and hemicellulose solubilization and lower cellulose content (79.7 %). For the alkali pretreatment, the best condition used to attain the highest enzymatic conversion of the group (72%) was 120º, 30 min and 4 % (w/v). The substrate resulted in 75% of cellulose after a removal of lignin and hemicellulose of around 81.3 % and 83.9 %. The best condition for hydrothermal pretreatment (195° C, 10 min) yielded a lower removal of lignin and hemicellulose (3% and 80%) and a enzymatic conversion of 66 %.
With the conditions used here, the organosolv pretreatment was the process with highest standard deviation for the enzymatic conversion: ranging values between 88.20 - 12.20 %. The average of this group (19 samples) was 36.37%. The ratio: maximum/minimum % in enzymatic conversion yielded 9.28 and the high dispersion of the population indicates the validity of the whole group of samples for future structural analysis and their effect in enzymatic saccharification.