Ethanol productivity of a non-GMO yeast newly screened for mixed sugars in cellulosic biomass

A non-GMO yeast newly screened (Candia intermedia sp. strain 4-6-4T2) can ferment both pentose (C5) and hexose (C6) sugars simultaneously, in cellulosic biomass. When the diluted sulfuric acid and enzymatic saccharification was performed to recover C5 and C6 sugars in agricultural residues such as sugarcane bagasse, 4-6-4T2 produced 50 g/l ethanol with 120g/l sugars (40g/l of xylose and 80g/l of glucose) in 48h without producing xylitol or glycerol. At the initial condition of pH adjusted to 5, pH control was not required for the yeast through the fermentation period. In addition to monosaccharides (xylose (C5), glucose, mannose and galactose (C6)), the yeast could ferment disaccharides (cellobiose, maltose, trehalose and sucrose). Using this yeast, we will show the evaluation of ethanol productivity for the mixed sugars in cellulosic biomass.