14-28: Obtaining and characterization of new glucoamilase of native Aspergillus spp. for sacharification of starch liquefied of cassava

Monday, April 29, 2013
Exhibit Hall
Luis J. Rueda, School of Chemistry, Universidad Industrial de Santander, Bucaramanga, Colombia, Andres M. Rueda, Grupo de investigación en bioquímica y microbiología, Universidad Industrial de Santander, Bucaramanga, Colombia, Clara Sanchez, School of Bacteriology and Clinical Laboratory, Universidad Industrial de Santander, Bucaramanga, Colombia and Daniel R. Molina, Grupo De Investigación En Bioquímica y Microbiologia, Universidad Industrial de Santander, Bucaramanga, Colombia
The obtainment of glucose syrup from cheaper sources of starch, that not affect the alimentary security, it is a priority in the biofuels production. The most efficient and ecology alternative for production of this syrups is the enzymatic hydrolysis of starch. The works propose, was screening and obtaing an enzymatic extracted from native filamentous fungi, to be used in the production of glucose syrups through the saccharification of cassava starch. It was made the screening of filamentous fungi and, it was choose a native culture of Aspergillus spp from a soil of cassava (Manihot sculenta). To the isolation selected, was made growth kinetic, evaluated maltose consume, production of protein, biomass and glucose. The enzymatic extract obtained, was made electrophoresis of protein and specific activity. Further, the best parameters were determined for enzymatic activity (PH, temperature, agitation, and enzymatic co-factors). Finally, it was evaluated thermic stability, and saccharification of liquated cassava starch, using previously established conditions. The used methodologies were: DNS for maltose, Bradford for protein, dry weigh for biomass and glucose oxidase for glucose. The kinetics parameters were determinate by an experimental design 33.