Paper: The Chitotriosidase Activity Identified in Pancreatin from Porcine Pancrease (35th Symposium on Biotechnology for Fuels and Chemicals (April 29-May 2, 2013))

Monday, April 29, 2013

Exhibit Hall

Jeen-Kuan Chen, Environment and Biotechnology Department, Refining and Manufacturing Research Institute, CPC Corporation, Chiayi, Taiwan, Chia-Rui Shen, Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Kweishan, Taoyuan, Taiwan and Chao-Lin Liu, Department of Chemical Engineering and Graduate School of Biochemical Engineering, Mingchi University of Technology, New Taipei, Taiwan

The versatile biopolymers, chitin and chitosan oligosaccharides, are mainly produced with enzymes. However, it costs more because the amount of chinases and chitosanases are limited. Fortunately, a plenty of commercial enzyme can hydrolyzed chitin and chitosan as usual substrates to produce long chain chitin or chitosan oligosaccharides. Herein, a platform to screen enzymes posing chitinase and chitosanase activity was applied on single gel with glycol chitin or glycol chitosan as substrate. An SDS resistance chitinase and chitosanse activities were observed in pancreatin. Its chitotriosidase existed by the optimal hydrolysis pH of 4 and the substrate specificity assay. The activity was thermal unstable but independent of 2-mercaptoethanol. It is the first time to identified chitotriosidase in hog. And the oligochitosacharrides can be mass production with pancreatin in low cost.

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