17-35: Comparison of solid-state and submerged fermentation for production of cellulolytic enzymes by marine-derived fungus Aspergillus niger using agro-industrial residues

Among the global trends for scientific and technological advancement in the area of biofuel production, highlights the importance of using agro-industrial residues as feedstock for bioethanol production. The biggest challenge of this research is to obtain high cellulolytic enzymes production at low cost. Several studies have shown that manipulation of culture conditions can result in increased production of cellulolytic enzymes. This study evaluated the production of cellulolytic enzymes by marine-derived fungus Aspergillus niger after growing in sugarcane bagasse (SCB) and wheat bran (WB), supplemented with Czapek-Dox or nutrient media. The cultivation was carried out by solid-state fermentation (SSF) and submerged (Fbm) in different incubation times (5-14 days) at temperatures of 28 and 37ºC. The determination of cellulolytic activity (FPase, CMCase and β-glucosidase) was quantified by the DNS method and glucose oxidase (GOD). Analyzing the results of full factorial design, it was concluded that the highest yields of enzymes FPase (6.4 U/g-ds), β-glucosidase (10.0 U/g-ds) and CMCase (115.2 U/g-ds) were obtained in medium containing SCB and WB supplemented with nutrient medium after 5, 11 and 14 days, respectively, at 28°C by SSF. Thus, this study confirms that the composition of the culture medium, as well as, the types of substrates used in SSF technology can promote increased production of enzymes in industrial level with low cost.