17-47: EFFECT OF CHEMICALS AND PHYSICAL PRETREATMENTS OF SUGARCANE BAGASSE IN ENZYMATIC HYDROLISIS

Monday, April 29, 2013
Exhibit Hall
Maira Prearo Grimaldi1, Sandra Regina Pombeiro Sponchiado2 and Marina Paganini Marques2, (1)Biochemistry, Universidade Estadual Paulista Julio de Mesquita Filho, Araraquara, SP, Brazil, (2)Biochemistry and Chemical Technology, Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara, SP, Brazil
Due to the growing demand for alternative sources of fuel, ethanol production from lignocellulosic materials became an interesting option. This process allows the increase of ethanol production without the need of growth of the planted area of the cultivars used. In order to produce a viable and economical cellulosic ethanol it is necessary to fractionate the biomass to provide the largest possible amount of carbohydrates to ethanol fermentation process, but this biomass needs a pretreatment to disrupt the recalcitrant structure of the material. In this sense, the main objective of this project was to evaluate the methods of alkaline pretreatments, using calcium hydroxide, and hydrothermal ,using water, in sugar cane bagasses in natura (obtained directly from the mill) and exploded (subjected to steam explosion). We tested different times (7, 30 and 60 minutes) at a temperature of 120 °C in an autoclave. Complete factorial designs were performed to examine the influence of calcium hydroxide and hydrolysis time performance by quantifying the release of glucose and total reducing sugars (TRS). Thus were analyzed the chemical composition of the pretreated bagasse to evaluate a possible correlation between changes in material structure and efficiency of hydrolysis. The results showed that the best yields were obtained with the in natura bagasse pretreated with calcium hydroxide at all times tested. This material increased the yield of hydrolysis 3.2 to 4.7 fold compared to untreated bagasse in natura.