Monday, April 29, 2013
Exhibit Hall
Cellulases (3.2.1.4) have a wide range of industrial applications such as textile, laundry, pulp and paper, fruit juice extraction, and animal feed additives as well as in bioethanol production. The cellulases have great potential in saccharification of lignocellulosics to fermentable sugars which can be used for production of bioethanol, lactic acid, and single cell protein. The efficient degradation of lignocellulosic biomass requires the synergistic action of the cellulolytic enzymes endoglucanase (EG), cellobiohydrolase (CBH) and beta-glucosidase (BGL). Cellulase yields appear to depend on a complex relationship involving a variety of factors like pH, temperature, incubation period, cations, carbon, and nitrogen sources. To establish a successful fermentation process, it is necessary to make the microorganism for overproduction of the desired metabolite. An elaborate investigation is therefore, required to establish the optimum condition to scale up enzyme production in an individual fermentation process. The main purpose of this study is production of cellulase enzyme by optimizing the production medium. We investigated effects of cellulase enzyme production by various conditions (temperature, pH, incubation period, carbon source, nitrogen source) from the brown-rot fungus Fomitopsis pinicola. After optimization of cellulose activity, we study about the large-scale production of cellulolytic enzyme.