Healthy tamarind seeds were germinated and endosperms were collected every 48hrs from 3rd to 31st day. A 20% extract of the fresh endosperm were prepared in different buffer systems. Maximum galactoxyloglucanase activity was extracted with 0.05M acetate buffer pH 5.5 containing 0.5M NaCl. The activity was determined using tamarind xyloglucan (amyloid, Megazyme, Ireland). The specific activity increased 15–fold during germination reaching maximum by 23rd day. Km of the enzyme was 66.7 mg%. The enzyme had optimum pH and temperature of 5.5 and 45°C and stable between pH 4.0 to 6.5 and 10°C to 50°C, respectively. Electrophoretic analysis of the galactoxyloglucanase activity during germination using grams iodine revealed five isozymes. Major band 2 was present on all the days while band numbers 3 & 4 appeared from 17th day onwards and band number 5 from 23rd day onwards.