Monday, April 29, 2013
Exhibit Hall
Our previous study of a co-hydrolysis process, which applied dilute acid pretreatment, directly followed by enzymatic saccharification without detoxification and liquid-solid separation between these two steps, along with oleaginous fungal (Mortierella isabellina ATCC 42613) lipid accumulation on corn stover showed promising results. However, additional chemicals were added for pH adjustment. Here, we introduced a combined hydrolysis process that first mixed the dilute acid and dilute alkali pretreated corn stover at 1:1 (w/w) ratio, and then directly applied enzymatic hydrolysis on the pretreated fiber without pH adjustment. In addition, we also increased the solid loading as it has better economic advances and more industrial interests. A combined hydrolysis with high solids loading (15% w/w) was studied and compared with co-hydrolysis as well. The oleaginous fungus Mortierella isabellina was selected and applied to the co-hydrolysate and combined-hydrolysate media to accumulate fungal lipids. Fungal cultivations on the hydrolysates from combined hydrolysis exhibited comparable cell mass and lipid production to the hydrolysate from co-hydrolysis, which indicated that the integration of combined-hydrolysis with oleaginous fungal lipid accumulation for biodiesel/biojet production was technically feasible and promising.