Monday, April 29, 2013
Exhibit Hall
Industrial biotechnological processes have been developing several bioproducts using glycerol as substrate.The aim of this study was to produce biosurfactant and lipids using the yeast Yarrowia lipolytica IMUFRJ 50678. Screening was performed by using a complete 33 factorial experimental design with these input factors: glycerol content, pH and inoculums content. The emulsification index and surface tension were chosen as the response variables. The experiments were conducted during 96h in flasks containing 100mL of Mineral Broth added with three different concentrations of glycerol as sole source of carbon and energy. The best condition (30 g/L of glycerol, pH=6 and 30% of inoculum) resulted the major emulsification index within 48h (64,2%) as well as a decrease in the surface tension (40,1%). Approximately 70% of glycerol was consumed within 48h. The yeast also yielded 0,67g/g and 42,2% of lipids, determined by dry weight at an interval of approximately 100h. Results highlighted the biotechnological potential of Yarrowia lipolytica IMUFRJ 50678 to produce with industrial interests.