13-24: Optimization of NaOH pretreatment and hydrolysis with Streptomyces AOA40 of wheat straw for production reduced sugar

Science and technology has rapidly expenden and used for human benefits over the last 20 years. Humanity can solve some problems with the help of developing technology. But, they faced with fundemantal problem such as environmental distortion from increasing population and consumption of energy, raw material acarcity, nutrient deficiency, and waste management. Therefore, the main theme in our research covers the conversation of wheat straw that are abundant in Turkey. For this purpose, Actinobakteria members to be used for hydrolysis were isolated from soil in Turkey province and lignocellulose degrading potential of each isolate was tested. İsolate has a maximum lignocellulosic degredation potential and codded as AOA40 were identified as Streptomyces sp. İncubation condition of maximum production of the lignocellulose degrading enzymes such as endoglucanase, endoxylanase, β-glucosidase, β-xylosidase, α-L-arabinofuranosidase ve peroxidase from Streptomyces AOA40 was determined. Extracellular enzymes produced under optimum conditions were used for hydrolyse to lignocellulosic material. Optimal conditions were determined for hydrolysis of wheat straw pretreated  with NaOH. Fort his purpose, parameters such as pretreatment temperature (40, 50, 60, 70, 80 ⁰C), pretreatment time(4, 8, 12, 18, 24), chemical rate (0.5, 1, 1.5, 2, 2.5 %) for pretreatment and hydrolysis time ( 2, 5, 10, 24 hours) were optimized. Finally, pretreatment with 1,5% NaOH at 40 ⁰C for 12 hours and hydrolysis for 24 hours were identified as optimum conditions for maximum reduced sugar production.