Monday, April 30, 2012
Napoleon Ballroom C-D, 3rd fl (Sheraton New Orleans)
Hemicelluloses and starches are the most polysaccharides found in the entire world over. In plants, the xylans or the hemicelluloses are situated between the lignin and the collection of underneath cellulose fibers. Starch is an easily accessible source of energy. It is in plant cells or seeds, usually deposited as large granules in the cytoplasm. Hemicellulolytic and amylolytic enzymes from microorganism have attracted a great deal of attention in the last decade, particularly because of their biotechnological characteristics in various industrial processes, related to food, feed, ethanol, and paper industries. A microbial screening of xylanase and amylase producer was carried out in Brazilian Cerrado. A fungus Neosartorya spinosa strain P2D16 show good xylanase and amylase producers. Thus, N. spinosa strain P2D16 was cultivated on solid-state fermentation using as substrate source wheat bran, wheat bran plus sawdust, corn straw, corncob, cassava bran, and sugar cane bagasse as carbon source. Wheat bran is the best substrate for enzymatic production among the mentioned substrates. Xylanase, β‑glucosidase, glucoamylase, CMCase and amylase enzymatic activities were determined. The best xylanase, β‑glucosidase, glucoamylase activities in the analyses present in the paper were 8.60 U ml-1 (86 U.g-1), 13.9 U ml-1, (139 U.g‑1) and 10.25 U mL‑1 (102.5 U.g‑1). These crude enzymes were characterized and shown a good themostability.