7-01: A multiplexed, high-throughput screening pipeline for lignocellulosic enzyme discovery and evolution

We demonstrate the integration and application of a nanoliter-scale acoustic sample deposition and nanostructure-initiator mass spectrometry (NIMS) analysis platform to rapidly detect and characterize glyco- and lignolytic enzyme activities and substrate specificities from complex environmental samples and crude cell lysates.  By coupling the analysis platform with single cell sorting, we were able to quickly screen large libraries of mutant b-glucosidases for enhanced thermostability and ionic liquid tolerance.  We discuss the results from our screen and present future strategies to enhance throughput by utilizing cell free protein synthesis and high-density microplates.