9-27: Efficient production of 1,3-propanedion from crude glycerol by constitutive expression of 1,3-propanediol oxidoreductase gene in engineered Klebsiella penumoniae

Tuesday, May 1, 2012
Napoleon Ballroom C-D, 3rd fl (Sheraton New Orleans)
Baek-rock Oh1, Sun-Yeon Heo2, Jeong-Woo Seo2, Chul Ho Kim2 and Don-Hee Park3, (1)Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials(CNU), Molecular Bioprocess Research Center(KRIBB), Chonnam National University, Gwangju, South Korea, (2)Microbe-based Fusion Technology Research Center, KRIBB(Jeonbuk Branch Institute), Jeonbuk, South Korea, (3)Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, School of Biological Science and Technology, Chonnam Nationnal University, Gwangju, South Korea
Klebsiella pneumoniae is one of the well-known microorganisms, which produce 1,3-propanediol (1,3-PD) from glycerol. In this study, we developed an efficient process for 1,3-propanediol (1,3-PD) production from glycerol by genetic engineering of K. pneumoniae AK mutant strains, in which by-products formation was eliminated but IPTG induction was necessary for maximal production of 1,3-PD. A series of recombinant strain was designed to constitutively express dhaB and/or dhaT gene using bacteriophage T5 PDE20 and rho-independent transcription termination signal of Rahnella aquatilis levansurcrase gene. Among them, AK/pConT expressing dhaT alone was the most proper for 1,3-PD production. Fed-batch fermentation showed efficient production of 1,3-PD from both pure or crude glycerol without by-products formation. Further engineering to enhance production level would provide an economical biological process for 1,3-PD production.
See more of: Poster Session 2
See more of: General Submissions