Polyphosphate is a candidate for renewable phosphorus fertilizer. Many microorganisms are reported to synthesize and store polyphosphate in the cells as phosphorus reserve and energy resource. While bacteria are well studied in polyphosphate accumulation and widely implemented in P removal process in wastewater treatment plants, Fungi are less investigated and their ability to accumulate intracellular polyphosphate is rarely reported. Compared with bacteria, filamentous fungi have the advantage of being easy to harvest and thus show promise in phosphorus recovery. In this study, several filamentous fungus species are evaluated for their potential in polyphosphate accumulation. Besides, the optimal conditions of polyphosphate accumulation are investigated. This research provides a new possibility of P removal & recovery. The phosphorus recovered in the form of polyphosphate, which has high purity and P density, can be transported to P deficient area with relatively low cost, providing a solution to the problem of P fertilizer industry.