Monday, April 30, 2012
Napoleon Ballroom C-D, 3rd fl (Sheraton New Orleans)
Biodiesel is a transesterification, where glycerin is separated from fat or vegetable oil. The process generates glycerin, esters, and residues (cake, bran, etc), that can be important sources of income for producers. Currently in Brazil, the main applications of biodiesel residues are organic fertilizer, power generation and animal feed. This study aimed to evaluate the profile of cellulolytic activity of Penicillium simplicissium grown on residues of the oleaginous plants [Crambe abyssinica (Ca), Helianthus annuus (Ha), Linum usitatissimum (Lu), Raphanus sativus (Ra), Guizotia abyssinica (Ga), Ricinus communis (Rc) e Lupinus albus (La)] used in the production of biodiesel as carbon source. The fungi were grown submerged on medium supplemented with 1% (w/v) of the biodiesel residues as carbon source at 120 rpm/28°C for 12, 24, 72 and 120 hours, respectively. Cellulolytic activity was measured for FPase, exo- and endoglucanase (DNS) and β-glicosidase (pNPG) in the crude extracts obtained by filtration. According to the induction curve, the cultivation times of 72 and 120 had the most significant results for the tested enzymatic activities. The substrates Ca, La and Lu showed significant results for FPase and endoglucanase, while exoglucanase was found to be relevant in the cultivation on La. Among the cellulolytic activities evaluated, that of β-glucosidase was the most expressive, especially when grown in Rc, Ha and Lu, with 3.52, 3.63 and 3.64 IU/ml, respectively. The crude extract of the cultivation of P. simplicissium with residues of Lu was selected for the process of purification and enzymatic characterization for endoglucanase and β-glucanase.