13-19: Stable second generation bioethanol production in high temperature fermentations

Tuesday, May 1, 2012
Napoleon Ballroom C-D, 3rd fl (Sheraton New Orleans)
Rasmus Lund Andersen, Karen Møller Jensen, Marie Just Mikkelsen, Thomas Kvist and Mads Pedersen, BioGasol ApS, Ballerup, Denmark
Thermophilic ethanol production from diverse biomass sources is of great potential as part of the future renewable energy supply. BioGasol ApS has developed a stable, cost-effective and scalable fermentation for processing pretreated lignocellulosic materials in high DM fermentations without the use of any cost enhancing detoxification steps. The center in this anaerobic high temperature fermentation is the metabolically engineered production organism PentocrobeTM 411, which has ethanol yields close to the theoretical limit and conversion rates higher than 95% on both C5 and C6 sugars.  

Traditionally, ethanol production is performed in batch or fed batch fermentations. Running a continuous fermentation will have profound impact on the feasibility and economy of the future production scale as costly downtime is circumvented and inhibitory compounds generated in the pretreatment are continuously processed by the adapted organism. PentocrobeTM fermentation in batch- and continuous modes are compared regarding stability, nutrient demand and productivity.  

Earlier studies have investigated the inhibitory effect of both the substrate related inhibitors such as 2-furaldehyde and 5-hydroxymethylfurfural (HMF) and the product ethanol concentration. In the present study the combined effect of temperature, substrate and production inhibition is investigated in continuous reactors, as it is of major importance in the scaling process.

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