Traditionally, ethanol production is performed in batch or fed batch fermentations. Running a continuous fermentation will have profound impact on the feasibility and economy of the future production scale as costly downtime is circumvented and inhibitory compounds generated in the pretreatment are continuously processed by the adapted organism. PentocrobeTM fermentation in batch- and continuous modes are compared regarding stability, nutrient demand and productivity.
Earlier studies have investigated the inhibitory effect of both the substrate related inhibitors such as 2-furaldehyde and 5-hydroxymethylfurfural (HMF) and the product ethanol concentration. In the present study the combined effect of temperature, substrate and production inhibition is investigated in continuous reactors, as it is of major importance in the scaling process.