9-17: Characterization of a newly isolated Clostridium sp. strain S1 capable of producing butyric acid from galactose

Tuesday, May 1, 2012
Napoleon Ballroom C-D, 3rd fl (Sheraton New Orleans)
Kyung Min Lee1, Okkyoung Choi1, Ki-Yeon Kim1, Han Min Woo1, Sung Ok Han2, Byoung-In Sang3 and Youngsoon Um1, (1)Clean Energy Research Center, Korea Institute of Science and Technology, Seoul, South Korea, (2)School of Life Sciences and Biotechnology, Korea University, Seoul, South Korea, (3)Department of Chemical Engineering, Hanyang University, Seoul, South Korea
Galactose is a main carbohydrate of red seaweed, which is considered as a candidate feed stock for microbial fermentation. A newly isolated bacterial strain, designated as Clostridium sp. S1, was capable of producing butyric acid from galactose as a sole carbon source. On the basis of polyphasic taxonomy, the isolate was closely related to Clostridium tyrobutyricum ATCC 25755T (95.7%). In batch cultures, strain S1 produced 3.12 g/L of butyric acid from galactose 11.9 g/L within 3 days. When 50 mM sodium lactate and 100 mM 2-(N-morholino) ethanesulfonic acid were added simultaneously, butyric acid production was increased up to 9.52 g/L. Interestingly, in a culture of Clostridium sp. S1 in mMRS medium, only butyric acid was produced as liquid bioproducts. These results suggest that the strain S1 can be a candidate for producing butyric acid from galactose, a main hydrolysate of red seaweed.
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