To find a microorganism with a high potential for producing 1,3-propanediol, we screened hundreds of microorganism using the colormetric assay. Finally five microorganisms were isolated from soil samples of garden and industrial area, which have higher yield more than control. The positive colonies that exhibited deep red color were picked up with sterile toothpick and transferred onto the new LB plates and grew overnight. The detection of 1,3-propanediol producer in aerobic condition, reduced the product cost and made the biological method of 1,3-propanediol production more attractive
Medium composition were optimized for 1,3-propanediol concentration from Klebsiella pneumoniae AJ4. Response surface methodology was applied for the significant fermentation factors identified by one-factor-at-a-time approach. The optimal media constituents were consisted of 108 g/L glycerol, 2.74 g/L peptone and 4.35 g/L ammonium sulfate. In this optimized medium, the maximum 1,3-propanediol concentration of 52.34 g/L.
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